FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11125769
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC232920.20935182098423938No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG210490.18919141679105506No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC183070.16454592936452303No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC163910.14732464785130808No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG132710.11928164246444448No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT132140.1187693183275691No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA122180.10981712814637802No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC118640.10663532561209926No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG114560.10296816336920173No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG113130.10168285895563714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA36650.052.7416464
GCGACAT38300.050.3452645
CTGCGAC45600.042.181643
GCTGCGA51750.037.4459232
TAGCCCC40850.031.6316171
TAGCCCA46150.029.0283261
CACGAAT34400.027.4742575
TAGGCCC35650.027.050861
TAGCCGC12050.026.4138871
CGACATC77650.025.7487646
GGCTGCG79700.024.676671
TAGGCCA45350.024.6171
TCACGAA39550.023.8968074
TAGCCCG11050.023.6453061
GTGTGCT112550.023.5454168
ACATCTG84400.022.958078
GCGCGGT35650.022.5134946
CATCACG43150.022.2343962
TTGATCG44900.022.05681422-23
TGATCGC45450.021.47888624-25
Produced by FastQC (version 0.11.5)