FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14383864
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC461960.3211654392727851No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC165510.11506643833673622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGAC30100.062.4806333
TGCGACA30600.061.6149024
GCGACAT31050.061.028055
CGACATC32550.057.9238936
GCTGCGA33600.055.9722332
CTAGGCG93200.049.7848137
TAGGCGG93450.049.6516238
GGCTGCG41950.045.96061
GCTAGGC116050.040.1869056
GGCTAGG131450.036.45435
CGGTCGA68100.034.2843468-69
ACCTCGT35050.033.874295
GACCTCG35150.033.6426934
TCGGTCG69800.033.00697368-69
CGCTCGG71800.032.912964-65
CTCGTGA36700.032.7395487
CGTGATC37200.032.1718339
TGCGCGC73700.032.1309760-61
CCTCGTG38000.031.8694696
GGCTGGC151500.031.705981
Produced by FastQC (version 0.11.5)