FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21305398
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC844990.3966084088173335No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC546070.2563059371150917No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT418210.19629297701925116No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT361500.16967530951545706No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC271550.1274559620993703No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT86800.064.179845
TGCGACA87050.063.8864024
CTGCGAC94600.059.091923
CGACATC104900.053.2417346
GCTGCGA105750.053.0859832
GGCTGCG138200.041.9036751
CTAGGCG266700.034.4748737
ACTCGGG238650.034.0083051
TAGGCGG270700.033.983248
GCTAGGC286950.032.2405936
CTCGGGA253700.032.143462
TGGCTAG302750.031.0443064
GGCTAGG307550.030.5906775
TCGGGAG270150.030.2740783
GACATCT202300.028.006897
CTGGCTA345500.027.341953
GGCTGGC368900.026.7308621
TTGATCG105950.026.68437822-23
ACATCTG213400.026.3945148
AGGCGGG352100.026.3291729
Produced by FastQC (version 0.11.5)