FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17100877
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1382900.8086719762968881No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT1009630.590396621179136No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG1003100.5865781035674369No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA871090.5093832322166869No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC807070.47194655572342875No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC802680.46937943592015774No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG775350.45339779942280156No Hit
CCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGA732160.4281417847751317No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG712690.41675640377975937No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT637360.3727060313924251No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG625890.3659987730453824No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG565540.33070818531704543No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT523920.3063702522391103No Hit
CCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCAT497780.2910844864856931No Hit
CCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACT495140.2895407060117443No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTT488500.28565786421363065No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT483460.282710646945183No Hit
GGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGG480880.28120195239109663No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG414610.24244955390299575No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG408520.23888833303695478No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG399830.23380672231020663No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT360320.21070264408076847No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG359190.21004185925669194No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC354060.20704201310845052No Hit
AGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGAT344230.20129376990431544No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG339860.19873834540766536No Hit
GCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCG338290.19782026383793067No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG315570.1845343955166744No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA313430.1832829977082462No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC311420.18210761939285336No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC299670.17523662675311913No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG294100.1719794838592196No Hit
CCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGGCCGGCGCGCGGC291690.1705701994114103No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG289670.16938897344270706No Hit
CCCCCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGG285900.1671844081446817No Hit
GACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGC283390.16571664716376827No Hit
CGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGC275910.16134260248758003No Hit
CGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGTAGTAGCG272240.15919651372265878No Hit
AGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGA263400.1540271881962545No Hit
CGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCG256040.1497233153597912No Hit
CCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGT254670.14892218685626474No Hit
CCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAAT249650.14598666489443787No Hit
CAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGG247150.14452475156683486No Hit
CGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTAT239900.1402852029167861No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC238590.1395191603331221No Hit
CCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATT236860.1385075163104208No Hit
CCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATTTAAAGTT232070.13570649037473342No Hit
CCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT230990.13507494381720891No Hit
CATGCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGC219630.12843200965658078No Hit
AGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCG217300.12706950643525475No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG216270.1264671981442823No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA214410.12537953462854565No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA213980.12512808553619795No Hit
TGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCG201900.11806412033722014No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC199560.1166957694625837No Hit
GGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGC193720.11328073992930304No Hit
GCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT193060.11289479481081584No Hit
CCATGGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTC187770.10980138620960785No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGG178610.10444493577727036No Hit
TCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGA178550.1044098498574079No Hit
CATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCAC178320.10427535383126842No Hit
CCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCG177950.10405899065878317No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG175630.10270233509076757No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAAT173600.10151526146875392No Hit
CCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATA173010.10117024992343959No Hit
CGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTC171240.10013521528749667No Hit
CCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCA171100.10005334814115088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCCA121800.047.695671
TGCGACA17500.047.488454
TAGCCCC44500.046.2197571
TAGCCGC15300.042.8437461
GCGACAT19500.042.612485
ACCGCCA93950.041.9138451
ACCTACG131450.040.0645452
CACGAAT237050.039.159775
CCTACGG136900.038.8858343
TCACGAA237650.038.666174
TAGCCCG10500.038.4529271
TACGGAA137300.038.2833945
CATCACG240400.038.1054422
ATCACGA241300.037.9436343
ATACCGC10650.037.911341
TAACGCT12650.037.1744421
CGGAAAC150700.035.629347
CACCTAC148350.035.0611651
ACGGAAA153000.034.6593326
AATGGGG269350.034.6328939
Produced by FastQC (version 0.11.5)