FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17100877
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2535471.4826549538950546No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA1815671.0617408686115923No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1257110.7351143453052145No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1182020.6912043165973301No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1129580.6605392226375291No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC900620.5266513524423337No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA869550.5084826936068834No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG835910.48881118787065714No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG821070.4801332703580056No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG702620.4108678168961744No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC603070.35265442819102205No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG534150.31235240157566185No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG532790.31155712072544584No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN518670.303300234251144No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG487180.28488597397665627No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC434130.2538641731649201No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA430380.2516713031735156No Hit
GCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATG424650.24832059782664948No Hit
GGTACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC424330.2481334729207163No Hit
GGCATGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC421170.24628561447462607No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG405320.2370170839776229No Hit
GGCGCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC398120.2328067735941262No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG384780.22500600407803648No Hit
GGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGC369220.21590705552703526No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA349060.20411818645324448No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA335200.1960133389650133No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT324500.18975634992287238No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC320870.1876336517711928No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA319730.1869670192938058No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC319680.18693778102725375No Hit
CAGCCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG318020.18596707057772532No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC308540.18042349523945467No Hit
GGCCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCG305370.17856978914005406No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC290580.16992110989395456No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG282610.16526053020555612No Hit
GCCCCGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA276520.16169930933951515No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA273820.16012044294570388No Hit
CAACCCATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG267040.1561557340012445No Hit
GGCCCGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC262480.15348920409169658No Hit
GGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTG257260.15043672906366148No Hit
GGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTT248250.1451679934309802No Hit
GCCCTGTCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG248040.14504519271146152No Hit
GATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGC247930.144980868525047No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA247200.1445539898333869No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA239010.1397647617721594No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA238460.13944314084008674No Hit
AGCCCTGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCG234930.13737891922151127No Hit
CGACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG234700.1372444231953718No Hit
ACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGA216450.12657245590386973No Hit
GGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTG215090.12577717505365368No Hit
GTCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA197000.11519877021511819No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG196110.11467832907049154No Hit
GCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACGGA192130.11235096305294752No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC184780.10805293786979463No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA180310.10543903684004043No Hit
GGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAG179980.10524606428079682No Hit
GGGGTGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA176240.1030590419427027No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC173700.10157373800185802No Hit
CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT171750.10043344560632768No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC171360.1002053871272216No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT52150.082.5800862
GGTGTTA159500.080.0781252
TACGGTG54600.079.883083
GCTAGTA112900.079.453695
GATAGTA173550.079.173485
TGCTAGT112850.078.3937764
CGACCTA32650.077.687721
GTGCTAG115250.076.143133
GGTACGG57000.076.1427151
GGTGCTA116850.074.685992
GTGATAG165050.073.549563
TGATAGT170650.073.0011754
GGTGATA164250.072.125282
ATAGTAG223350.071.0293356
TGGTGTT178000.069.3786241
GACCTAT46900.069.3489762
GCCCCGT54300.068.535451
GCGCGGT61300.068.162342
CGCGGTG63050.066.23823
TTCGACG10050.065.819881
Produced by FastQC (version 0.11.5)