FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004914878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99494
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT6970.7005447564677267No Hit
NAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG5800.5829497256115946No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT3670.36886646430940556No Hit
NACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC3660.3678613785755925No Hit
NTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT3650.36685629284177945No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG3320.3336884636259473No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2650.26634771946047No Hit
NACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG2420.2432307475827688No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC2060.2070476611654974No Hit
NGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1720.1728747462158522No Hit
NGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1570.15779846020865582No Hit
NGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC1570.15779846020865582No Hit
NTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1470.14774760287052485No Hit
NGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1420.14272217420145938No Hit
NATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA1360.1366916597985808No Hit
NCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1220.1226204595251975No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG1160.11658994512231895No Hit
NAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT1090.10955434498562727No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)