FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588841
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129340.4996058081589406No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA91120.3519721759660018No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC73760.28491514156334824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65880.25447681027919444No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC63520.24536076182353414No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC62650.24200018463860856No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT61320.23686275055130848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60140.23230472632347834No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT56050.2165061508219315No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA55980.21623575955417887No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC48570.18761291249636422No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC46060.177917454181234No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC42260.16323907107466237No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT41500.16030339445334804No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC39310.151844010505087No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT38900.15026029022253587No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA36300.1402171859917237No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG34970.13507975190442364No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT34230.13222132993103863No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC32330.12488213837775282No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC31260.12074901471353398No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA30120.11634549978156247No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG30040.11603648118984518No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT29710.11476177949901133No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC29010.11205786682148498No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA28410.10974022738360525No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA28040.10831101639691275No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC27920.1078474885093368No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG27570.10649553217057363No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT27420.10591612231110369No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA26750.10332809160547134No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT26340.10174437132292019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA178600.067.4534451
GGTATCA95450.050.797491
TATCAAC268650.045.2819482
ATCAACG287000.042.490383
TCAACGC295200.041.451154
CAACGCA297550.041.003825
AACGCAG302600.040.4956636
ACGCAGA307850.039.6311577
CGCAGAG308450.039.534788
GCAGAGT321850.037.907269
CTATTGA36500.035.6912279
GCTATTG36450.035.413798
GGCTATT38900.033.6421177
GGGCTAT39700.033.563546
TTTAGGG40050.032.5282142
TCTCTGC19450.032.4187668
ATTTAGG42950.031.581461
AGGGCTA44750.030.7070125
AGAGTAC321950.030.4586610-11
CACTGTG34900.030.16887
Produced by FastQC (version 0.11.5)