FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588841
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA93060.35946587681514625No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC77030.29754627649979276No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT65380.2525454440809613No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC61960.23933489928504686No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC59480.22975532294181064No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT58980.22782395674357755No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA53000.20472481701270953No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC47890.18498625446676717No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC45900.17729941699779941No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT44030.17007610741640758No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT40700.15721320853617507No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC40120.15497282374622467No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC39300.15180538318112236No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC33910.13098525556416946No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA33520.12947878992954764No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT33120.12793369697096113No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG32930.12719977781563255No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA31580.12198508908040316No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG31240.12067176006560465No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA30560.11804510203600761No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC29550.11414374231557672No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC29060.1122510034413083No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT28500.11008787329928721No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC28220.10900630822827666No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA27730.10711356935400823No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG27520.10630239555075031No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT26380.10189888061877883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA163000.066.222711
TATCAAC236400.045.7619172
GGTATCA90600.045.7380681
ATCAACG250200.042.976123
ACGCAGA255800.041.8296667
CGCAGAG257900.041.488268
TCAACGC259000.041.4469644
CAACGCA258750.041.4361845
AACGCAG260750.041.2874376
GCAGAGT269850.039.6289449
TCTCTGC18700.036.2785648
CTATTGA38900.035.9505169
GCTATTG38750.035.782538
TTTAGGG41550.033.5306172
GGGCTAT43600.032.485846
GGCTATT43350.032.3987
TTCTCTG20650.031.9888177
ATTTAGG44900.031.2940941
CTGTGCA38250.030.8049569
CACTGTG37700.030.6235567
Produced by FastQC (version 0.11.5)