FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004914890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112869
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT15921.4104847212254916No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT8900.7885247499313363No Hit
NAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG7610.6742329603345472No Hit
NTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT6310.5590551878726665No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG4280.3792006662591146No Hit
NGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA4120.3650249404176523No Hit
NACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC3990.35350716317146424No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT3900.34553331738564175No Hit
NACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG3080.2728827224481479No Hit
NTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA2940.2604789623368684No Hit
NGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC2900.2569350308765028No Hit
NGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC2750.243645287900132No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC2430.21529383621720755No Hit
NGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT2340.20731999043138508No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA2260.20023212751065395No Hit
NGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1960.17365264155791227No Hit
NCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1890.16745076150227253No Hit
NCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1870.16567879577208977No Hit
NTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT1810.16036289858154143No Hit
NGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1730.1532750356608103No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC1720.15238905279571893No Hit
NAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT1600.14175725841462228No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG1570.1390993098193481No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1570.1390993098193481No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1530.13555537835898254No Hit
NAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG1420.12580956684297726No Hit
NATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA1410.12492358397788586No Hit
NTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT1220.10808990954114947No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1210.10720392667605808No Hit
NGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA1200.10631794381096668No Hit
NGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA1170.10365999521569254No Hit
NTGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGG1160.10277401235060114No Hit
NCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC1150.10188802948550976No Hit
NCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCC1140.10100204662041837No Hit
NGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGT1130.10011606375532697No Hit
NCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1130.10011606375532697No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)