FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6714556
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC20500.044.6897249
TTCCGAC21950.042.8244558
GTTTCCG23700.035.970086
TTTCCGA26600.035.6052177
ATAGCGC26300.032.8890894-95
ATTGCGC14350.032.4388739
CATAGCG31300.030.43610294-95
CCGTTTC28900.030.1920264
CTCCGTT23150.029.7347342
CGTTTCC27700.029.327815
CCGATCG32950.029.08696286-87
TCCGTTT28900.028.9774043
GTCCCGT8750.028.508491
GTTCCGT8450.027.713241
CATTGCG17200.027.1793638
TCACCCC66650.026.079359
GCTCCGT21900.026.035151
CGGCATA35700.025.52647290-91
GATCGGC38850.025.43742688-89
CACCCGA38150.025.10548682-83
Produced by FastQC (version 0.11.5)