FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249525
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120430.5353574643535858No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA68160.30299729942987963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60190.2675675976039386No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT55870.24836354341472086No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC55460.24654093641991087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51550.22915948922550317No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT49540.22022426956801994No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC47090.2093330814283015No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC43810.1947522254698214No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT39790.17688178615485492No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC39190.17421455640635244No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA38450.1709249730498661No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT34500.15336571053889153No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC33570.14923150442871272No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC31640.14065191540436314No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT29550.13136106511374623No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC28630.12727131283270912No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC26530.11793600871295051No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG26030.11571331725586513No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC24900.11069003456285215No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA24640.10953423500516775No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT23940.10642246696524822No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC23730.10548893655327235No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA23110.10273279914648648No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA22650.10068792300596792No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT22650.10068792300596792No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG22630.10059901534768452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA185650.054.69821
TCTCTGC13350.043.2220768
TATCAAC242650.041.9209332
GGTATCA110450.041.849451
ATCAACG248700.040.8533063
TCAACGC256200.039.773464
CAACGCA257350.039.595735
AACGCAG268950.038.0648846
ACGCAGA273800.037.1733477
CGCAGAG276850.036.8067938
GCAGAGT285950.035.677079
ACTGTGC36800.034.269148
CTGTGCG10950.033.138359
CTGTGCA27300.033.1204389
GGGCTAT33050.030.0580046
GGCTATT33200.029.92227
GCTATTG32800.029.7430258
CACTGTG31650.029.696047
GAGTACT72950.029.1117112-13
CTATTGA33950.028.9107489
Produced by FastQC (version 0.11.5)