FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2249525
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA74500.33118102710572234No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC68950.30650915193207456No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT51380.22840377413009413No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT47710.2120892188350874No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC44970.19990886965025953No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC44220.19657483246463145No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC40800.18137162289816738No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA40670.1807937231193252No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT37230.16550160589457774No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC35060.15585512497082718No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC33560.14918705059957102No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT32260.14340805281114902No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC29910.13296140296284772No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT28100.12491525988819863No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC26940.11975861570776053No Hit
GTATCAACGCAGAGTACGGGGGAATTCTGGACATTAATTAGGGCTGAAAG24790.11020104244229337No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA24540.10908969671375068No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA23210.10317733743790355No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACA23200.10313288360876186No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC22990.10219935319678598No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC22540.10019893088540914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141950.053.2234041
TCTCTGC14500.043.0985348
GGTATCA87200.040.4869081
TATCAAC191500.039.2033122
ATCAACG197550.037.7013323
CTGTGCA29900.037.223029
TCAACGC205700.036.2944034
CAACGCA207750.035.78825
ACTGTGC36750.035.6292348
ACGCAGA211950.035.181977
CGCAGAG213400.035.0018048
AACGCAG215000.034.765926
GCAGAGT219450.034.145339
CACTGTG32750.033.253887
ACAGCAC38000.029.1410883
TTCTCTG21900.028.5330167
ACACAGC37500.028.2595461
GCTATTG32400.028.1052598
CATGGTA5200.027.4779514
TAGGCAT12700.027.1858375
Produced by FastQC (version 0.11.5)