FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4093151
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111240.27177106341789004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC13750.044.908448
TCCGACC15050.041.344929
TTTCCGA17450.035.6580777
GTTTCCG15950.032.4583666
CGTTTCC17250.031.6789845
ACCCGAT20550.030.8519484-85
CCGATCG20850.030.63580786-87
ATAGCGC20300.030.5300594-95
CGGCATA20850.029.4969390-91
ATCGGCA21050.029.21667388-89
CGGGGAT36300.029.1800218
CCGTTTC19750.029.115434
CTCCGTT13900.029.062322
GATCGGC22750.027.86845888-89
TCCGTTT18900.027.6596363
CATAGCG23200.027.6349694-95
TCGGGGA39600.027.2278127
CGATCGG23050.026.78462286-87
GTTCCGT5700.026.6809521
GCTCCGT13700.026.3644811
Produced by FastQC (version 0.11.5)