FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14076350
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG319730.2271398480429941No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT235840.167543432779094No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC192350.13664763948040506No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC177080.125799656871277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA59900.046.269754
GCGACAT61450.045.374355
CTGCGAC72250.038.584513
CCTCGAC30950.037.2996641
GCTGCGA76700.036.285882
CCGACTC99850.035.3374985
CGACTCC99850.035.2264676
CCCGACT109000.033.3412064
GAACCCG106950.033.3111721
GACTCCC114700.030.7144137
ACCCGAC117250.030.3003373
ACTCCCT120900.029.5075848
GGTGTGC85800.027.762497
GGCTGCG99250.027.6962741
AACCCGA127500.027.6444552
TTGGACG32050.027.4366447
GCGCGGT34950.027.3893286
CTCGACC42150.027.1688042
GTGTGCT117500.026.6668958
CGCGTCG21350.026.1593312-13
Produced by FastQC (version 0.11.5)