FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14076350
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200930.14274296959083854No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA175110.12440014634475557No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG102100.042.4850588
TAGTAGC204100.039.2251667
GTACACC69000.039.2111057
AGTAGCA220500.037.4036838
GTAGCAA229250.036.7078259
CGTCGGT53800.033.774029
TCAGTAC129150.033.5886577
GGTACAC70150.033.4689526
CGGTACA59250.033.4163065
AGTACGA134100.032.958269
GTAGTAG159100.032.1741756
AAACGAG184800.031.12226322-23
ACGAGAA183700.030.77847124-25
CGAGAAC176900.030.74106824-25
GCTCTGA179900.030.6534238
CAAACGA183650.030.35000620-21
TCAGTCG157450.030.2748184-85
GGTAGTA130250.030.2083855
CGATCCT80400.030.0145242-43
CGAAGTG173400.029.90089840-41
Produced by FastQC (version 0.11.5)