FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427003
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG540310.995595543249193No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT230850.4253728991857937No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT192950.355536932631141No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC170630.3144092605071344No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA143470.2643632222056999No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG140030.2580245487242222No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC136220.25100409931595763No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA130000.23954289319537875No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC104160.1919291365786973No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG94980.17501372304382362No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG84020.15481841450981326No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT78420.14449964372601232No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC71730.13217239791465013No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG68880.12692088064075147No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA66400.1223511392936396No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT62630.11540439539097361No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT61120.11262201255462728No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA59560.10974749783628276No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT58580.10794171294911759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT10300.052.113855
TGCGACA10350.051.4031374
CTGCGAC11200.047.5020033
CGACTCC53050.043.424646
GTAGCAA41800.041.9328169
GCTGCGA13350.041.276292
CCGACTC56200.040.8246735
TATTCGT40000.040.259062
CTCACGT149500.038.8905569
GCTCACG153500.038.033258
TTCGTAC42400.037.9791954
AGTAGCA47150.037.5791638
TAGTAGC46450.037.429617
ACTCCCT62100.037.0996748
ATTCGTA43500.037.0188063
CCCGACT63450.036.8337064
ACGCATT32700.036.7523163
ACCCGAC63950.036.1743163
AACCCGA65250.035.8185922
TACGCAT34650.035.6446272
Produced by FastQC (version 0.11.5)