FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004914934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41775331
Sequences flagged as poor quality0
Sequence length101
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG8047541.9263856939876791No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC4791241.1469065319913323No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT4319301.0339355539756225No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG3548250.8493649038950764No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT2441590.5844573679140926No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1828710.4377487757068879No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1708170.40889442623446837No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1671720.4001691811849438No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1546440.37018019079250386No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1479510.35415877375094884TruSeq Adapter, Index 1 (100% over 49bp)
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA1453440.3479182486908362No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG1327150.3176874888196577No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC1267430.3033919707302858No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG1181490.2828200212225727No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC1151680.27568423096396294No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG1141490.27324499236164046No Hit
TACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCC1110090.26572859470580856No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA1093740.2618148016589025No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC1090620.26106794940774974No Hit
ACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCG1006140.24084548845346074No Hit
TGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCG969350.23203885565861826No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC964790.23094730236847194No Hit
TAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAG954440.2284697636507057No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG932740.22327531049364996No Hit
ACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCA916600.21941178634826375No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC893760.2139444448686714No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT831080.19894037464359052No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT804750.1926376118958818No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG790900.189322258152784No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT785220.1879626040545316No Hit
CCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGC754530.1806161631609813No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG730630.17489508341657425No Hit
TGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCA699700.16749119234985835No Hit
ATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGG696850.16680897154351693No Hit
ACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCA680810.16296938497028307No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA654840.15675279748232276No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC633960.15175463241691609No Hit
CAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGG633080.1515439817819756No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA586700.14044173581772457No Hit
CTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGT586060.14028853535594965No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT582840.13951774553264462No Hit
ACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGC575760.1378229654242596No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG569130.13623590439056005No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAG565870.13545553953839407No Hit
GTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCG557880.13354292752342287No Hit
CCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACAC551030.1319032038309882No Hit
AGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGT549650.13157286533528603No Hit
GCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGA544550.13035204915551715No Hit
ACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCC542850.12994511042892753No Hit
CACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTT539040.12903308892992374No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG537820.1287410505496653No Hit
CCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCG534250.1278864792238271No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG532240.12740533402356524No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA530110.1268954637367206No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA528980.12662496917139926No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT528340.12647176870962434No Hit
AATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCT524310.12550708455188542No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA523060.12520786489998129No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG520440.12458070050959022No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG519050.12424796825667284No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG510880.12229226861182739No Hit
ACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCC510870.12228987485461217No Hit
TACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACG504250.12070520757812787No Hit
CCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGC494950.1184790133679611No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCC487140.11660948898286408No Hit
ACCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGG485030.1161044062104499No Hit
CACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGG478360.11450777014788943No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG453180.10848028947993255No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC453010.10843959560727359No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT450600.10786270011840242No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC449360.10756587422371353No Hit
AACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCA441420.10566523099481845No Hit
TACCCACGTGTTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCC438730.10502131030392076No Hit
ACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA436970.10460000903403974No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC433150.1036855937778207No Hit
CAAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACG425250.10179452557778657No Hit
CACATGGAAGCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATT425150.10177058800563422No Hit
ACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGA424060.10150966846917382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)