FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004914937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004914937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404758
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA47840.19893893689094705No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC29640.12325564568243458No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT27420.1140239475240336No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC25960.10795265053697711No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC25930.10782789785916087No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC24520.1019645220017981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91500.059.347641
ATCAACG124100.043.1337933
TCAACGC124450.042.9167984
TATCAAC126450.042.8501432
CAACGCA126250.042.349435
AACGCAG127200.042.20286
CGCAGAG128350.041.8237958
ACGCAGA128700.041.57227
GCAGAGT137200.039.0826039
GGTATCA49850.038.5804331
AGAGTAC136550.030.39973810-11
TACGGGC27700.025.88686214-15
CAGAGTA140900.025.70210-11
AGTACGG159650.025.66538812-13
ACTGTGC22300.025.628
TACGGGT37900.025.27893614-15
TACGGGG44300.024.51496514-15
GGGCTAT24050.023.0138726
TACCGCA3655.456968E-1222.8274297
CTGTGCA22100.022.0817959
Produced by FastQC (version 0.11.5)