FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882603
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182920.47112723088093217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118520.3052591264159637No Hit
TTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102340.2635860529649825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72820.18755458644625783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCAA79550.070.245381
GTATCAA169450.057.114461
TATCAAC314850.048.77192
ATCAACG319100.048.191413
TCAACGC320900.048.0078854
CAACGCA323550.047.736065
AACGCAG330000.046.786046
TAGTACG21500.046.710351
GGTATCA72600.044.590031
ACGCAGA346200.044.4346927
CGCAGAG355600.043.2127658
TTACGGG30300.042.9579851
TGTATCA30900.042.4869881
GCAGAGT367400.041.8248759
AGAGTAC360750.032.10630410-11
AGTACGG248700.028.22041112-13
TATTACG5600.028.0523071
AGTACTT160550.028.00754212-13
CAGAGTA368400.027.82279210-11
GAGTACT156600.026.79733512-13
Produced by FastQC (version 0.11.5)