FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36831153
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28550.031.91499743
CGTATGC31750.029.05262844
CTCGTAT32850.026.36749642
GATCGGA36050.025.7252161
ACGATCT41450.021.27627837
TCACGAT42700.020.86755635
ACACGTC45000.020.54715313
TCTCGTA44050.019.91878541
CACGATC46700.018.74049236
GTATGCC55400.016.93444645
CACGTCT57100.016.66581514
TCGGAAG59900.016.5281723
ATCTCGT55400.016.08154740
CGTCTGA62300.014.98588416
ACGGACA64300.014.48477318
CACACGT66550.014.06285512
GTCCGAT18400.013.8238071
CGGACAG71700.013.02173719
TCACCCG75100.012.613663
ACACGGA78450.012.01554316
Produced by FastQC (version 0.11.5)