FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36831153
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACA67800.014.36982118
CGGACAG73650.013.38149219
TCACCCG75600.013.0401433
GTCCGAT19800.012.621921
CGGACAC80350.012.54189413
ACACGGA79200.012.44074816
CGCGTAT2654.8017682E-811.8945991
CGGCCCG92800.011.4892878
CACCCGG88200.011.2022524
CGCGATA2851.3164026E-711.059891
CGCCCTT39750.010.7617791
GACACGG93400.010.7175615
ACCCGGC92100.010.7019255
ATAGCTC92450.010.56163339
TAAGTCG11700.010.19320733
GGACACG102000.010.12242714
GCCCGGA100350.09.93009110
GATTGAC100100.09.86794126
CACGGAC99400.09.84560217
CCGGACA106900.09.72155812
Produced by FastQC (version 0.11.5)