FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30844420
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC440800.14291077608202715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG16350.020.22787343
CGTATGC17350.019.19170844
TATCTCG17550.018.2039439
CGGACAC137300.017.77861413
TCACCCG139500.017.6924513
ACACGGA138700.017.64782316
ACGGACA138550.017.58599518
CGGACAG142400.016.99993519
GGACACG143800.016.99060814
GCCCGGA145450.016.92204510
GACACGG145400.016.81914115
CGATGTA19350.016.62843934
CACCCGG158400.015.8372914
ACCCGGC163550.015.32498555
ACCGATG22800.015.00048132
GATCGGA22100.014.9737291
CACGGAC163950.014.82009617
CTCGTAT22450.014.63144342
GATTGAC175350.013.76977926
CACCGAT24800.013.70083531
Produced by FastQC (version 0.11.5)