FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30844420
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC446770.1448462963479294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG140350.017.7823433
CGGACAC141950.017.38503513
ACGGACA143450.017.1737218
ACACGGA145000.017.0198216
CGGACAG147300.016.75566919
GGACACG149150.016.56071714
GCCCGGA151350.016.36527410
GACACGG151400.016.34450515
CACCCGG164600.015.3675624
ACCCGGC162950.015.2611895
CACGGAC166850.014.73794717
CGCTCAA22400.014.0694841
CGCGAGA50300.013.8648876
GATTGAC182450.013.60204826
CCGGACA196750.013.10286912
GGATTGA194700.012.76906525
CGCCGGA119700.012.6691139
CCCGGAC204400.012.55815911
CCCTCGT42450.011.9317181
GCGAGAT60150.011.8935067
Produced by FastQC (version 0.11.5)