FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32836546
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC383290.11672664962995803No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGA330550.10066527703614138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG16250.025.05890743
CGTATGC19400.020.99006544
CTCGTAT18700.020.81332442
GATCGGA22650.019.9772031
ACGGACA119400.016.46839718
ACACGGA126450.015.62089916
CGGACAG126200.015.59886319
CGGACAC126650.015.47188713
CGATCGT3650.015.4189211
CACCCGG134650.014.8710994
ACACGTC31650.014.85603513
GCCCGGA132800.014.84073910
GACACGG139350.014.19097515
GGACACG143900.013.82043714
TCACCCG150650.013.6944863
ACCCGGC147200.013.435215
GGATTGA148850.013.34938425
GATTGAC148800.013.23288326
CACGGAC150400.013.04364517
ATAGCTC157400.012.42100139
Produced by FastQC (version 0.11.5)