FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32836546
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC369850.11263364910548143No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGA353950.10779148330643545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACA116350.016.41671818
CGGACAG124100.015.62746119
ACACGGA128450.014.97407216
CGGACAC127050.014.943850513
CACCCGG132950.014.7606074
TCACCCG138650.014.348523
GCCCGGA135200.014.34292910
GACACGG141000.013.78457315
ACCCGGC145400.013.388785
GGACACG146050.013.369328514
CACGGAC146350.013.18950117
TTCCTCG149750.012.90539618
GGATTGA152600.012.79797725
GATTGAC151600.012.71937226
CGCACTT157000.012.3847953
ATAGCTC158450.011.94209639
CCCGCAC177300.011.9227531
CCCTCGT51300.011.8924591
ACTTACT184800.011.8814216
TTCGTTC73350.011.7769939
Produced by FastQC (version 0.11.5)