FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185627
Sequences flagged as poor quality0
Sequence length125
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT93050.42573595586072094No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC85280.39018551655886385No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT74900.3426934239007845No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT50130.2293621006695104No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG49380.22593059108438904No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT47760.21851853038052696No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC46310.21188427851595903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42600.19490974443489215No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC41620.1904259052436669No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG40150.18370014645682908No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG35260.16132670396183796No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT33900.1551042332474846No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC32950.1507576544396642No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG32060.14668559639865358No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT30840.1411036741401895No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA30650.14023435837862544No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATG29720.135979286493075No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC29400.13451517573675656No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG29120.1332340788249779No Hit
GTATCAACGCAGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGT26140.11959954740676246No Hit
GTTCCGACCTGCACGAATGGCGTAATGATGGCCAGGCTGTCTCCACCCGA25400.11621379128277606No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG24970.11424639245397317No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG24960.11420063899283822No Hit
TTCCGGCACCGGGCAGGCGTCACACCGTATACGTCCACTTTCGTGTTTGC24220.11081488286885181No Hit
GCGTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACT23550.10774940097281009No Hit
GTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGT23020.10532446753265769No Hit
GTGTACCCGCGGCAAGACGGAAAGACCCCGTGAACCTTTACTATAGCTTG22740.10404337062087904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22150.10134391641391692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGAG16850.086.451326
CAGAGAA17700.082.635618
TAACAAG8350.081.175286
ACAGAGA18750.077.373817
AATACAG20200.076.529244
ATAGTTC6700.073.802991
GTATCAA114650.073.7878651
ATACAGA20100.073.064485
TGTAACA6700.067.444144
TCATGTA7000.067.235671
GGTATCA54900.064.6762161
TAAAAAG12300.064.291264
CATGTAA7500.064.213912
AAAGGTC12250.064.068317
AAATACA24200.063.6340873
AGAGAAA23050.062.939659
TATCAAC170200.051.143092
TAGTTCA11650.051.036342
AAGGTCA15500.050.6346328
ATCAACG181100.047.9007453
Produced by FastQC (version 0.11.5)