FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321322
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103200.781035962467892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64630.48913133967344824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43070.32596142348345064No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT19910.1506824226040284No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC19050.14417378958346264No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA18410.13933015570769275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18310.13857333791460372No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT18140.13728674766635235No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC16480.12472357230107421No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC15180.11488494099091667No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT14870.11253880583234063No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC14810.11208471515648721No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT14410.10905744398413104No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA14120.10686267238417281No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG13600.10292721986010979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33600.080.0234151
GTATCAA73400.064.897891
TATCAAC96550.049.3502432
AGCAACG1600.048.439891
ATCAACG100950.047.72963
CAACGCA101850.047.366245
AACGCAG107550.045.187756
TCAACGC107350.044.8840524
ACTTGCG1151.4295438E-841.3810238
ACGCAGA124250.038.77917
CGCAGAG124400.038.7323388
GCAGAGT130550.036.7710279
GTACTGG12350.034.7572441
ATAGACG1102.043468E-532.4464843
GAGTACT57450.029.56141312-13
GGTTCAC10850.029.0572996
TACTGGT10950.028.7919332
GTAGCGA1254.8914306E-528.5529089
ACAGCGT1055.91471E-428.3262968
CAGAGTA132100.028.1890610-11
Produced by FastQC (version 0.11.5)