FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321322
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65290.49412633710783593No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT21650.16385105220377774No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC20140.15242310352813318No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA17980.13607583919740987No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT17030.12888607016306397No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC17010.12873470660444614No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC16840.1274481163561948No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA15890.12025834732184888No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC15790.11950152952875984No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC14670.11102517024616256No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT14010.10603017281177486No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA13850.1048192643428324No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG13440.10171631139116732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.062.217451
GTATCAA42650.056.8464161
TATCAAC58150.041.489512
CAACGCA58150.041.08235
ATCAACG59200.040.452493
AACGCAG63750.037.9410256
TCAACGC66600.035.957774
ACGCAGA70250.034.0087747
CGCAGAG70400.033.8544588
GTACTGG13800.033.1568761
GCAGAGT73450.032.5295759
GGCGTCG3800.029.717638
GTCCTAT1254.9258786E-528.5235251
GTGATCG5350.027.7734858
ACTGGTT11950.027.3499643
GGTTCAC12200.026.7915426
TGATCGC5650.026.2987889
AGAGTAC72850.026.1905310-11
TACTGGT13200.026.1105522
TACGGGC4400.025.6603684
Produced by FastQC (version 0.11.5)