FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004915562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800495
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65610.36439979005773415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47300.26270553375599487No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG42320.23504647333094508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.17400770343711033No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT28650.15912290786700325No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG28180.1565125146140367No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC26410.14668188470392865No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA26170.14534891793645638No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT23960.1330745156193158No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT23790.1321303308256896No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA23360.1297420987006351No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA22920.1272983262936026No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT22190.12324388570920775No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC21130.11735661581953852No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA20540.11407973918283582No Hit
CCCTATAGAAGAGCTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC20470.11369095720898975No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT19950.11080286254613314No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT19920.11063624170019912No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC19090.10602639829602414No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA18820.10452681068261784No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA18410.10224965912151937No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT18300.10163871601976122No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT18150.10080561179009107No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA18120.10063899094415703No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCCG250.002067867771.305548
GGTATCA44200.064.6805041
GTATCAA79450.053.0399251
GGGCGCG350.00783787650.932539
CGTTTCA653.543397E-545.7201081
TAGCGTG705.4959637E-542.443779
TAACGCA705.4959637E-542.443774
ATCAACG101850.041.6035923
CAACGCA102650.041.215745
GTACTGG7550.040.9361531
TCAACGC104400.040.581784
AACGCAG109550.038.782496
TATCAAC113350.037.386822
GCTTGGT1209.370706E-734.671081
CGTAGCA350.002761793834.06997768-69
GGGACGT350.00280698133.9573820-21
GTACAGT700.002807565533.9550174
ACGCAGA125850.033.759417
CGCAGAG125900.033.7460068
GTACTAA1251.2893252E-633.2842371
Produced by FastQC (version 0.11.5)