FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004915657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422438
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182881.285679938246869No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT120570.8476292112556048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101330.7123684828442435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94520.6644929339626754No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA78010.5484246062042775No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG67260.47285013476861554No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58060.40817244758646776No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT57100.40142347153267843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55230.3882770285945679No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT53690.3774505461749475No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC41850.294213174844879No Hit
GCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTT39330.276497112703682No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC37290.2621555385893796No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37250.2618743312538051No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC36720.25814833405744225No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA36520.25674229737956944No Hit
TCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTG36140.25407082769161116No Hit
GGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGC33680.23677657655377599No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG29840.20978067233861863No Hit
GCCATGAGGACCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCA28660.2014850559391692No Hit
GCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGCGAGGGT28550.20071173576633922No Hit
TTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGG25970.18257386262178035No Hit
AGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAG25250.17751213058143836No Hit
GAGTACGGGTGCTCCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTC24920.17519217006294827No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT24630.17315341688003272No Hit
TTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCA24510.17230979487330905No Hit
TGCCTGGAGTGGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAG23270.16359236747049782No Hit
GTATCAACGCAGAGTACGGGGCTCCTTGCTATAGAAGACCTGGGACAGAG22930.16120210511811411No Hit
CTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAA22770.16007727577581587No Hit
TCTCTGGTCACCCTGCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGA22660.15930395560298585No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT22480.15803852259290035No Hit
GTATCAACGCAGAGTACGGGTGCTCCTTGCTATAGAAGACCTGGGACAGA22260.1564918822472403No Hit
GCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACC21590.1517816593763665No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC21370.15023501903070643No Hit
GGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGG21110.14840717134947182No Hit
CTCTGGTCACCCTGCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGAC20980.1474932475088545No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT20930.14714173833938632No Hit
CTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGC20870.14671992733602449No Hit
GAAGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTG20710.14559509799372625No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20340.14299393013966163No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT20010.14067396962117154No Hit
CTTTGGAGCCAAGCTTTCGTCCCATGCAAGGGAAACAACCACTTCTGGGA19960.14032246045170335No Hit
GGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTT19450.13673706692312776No Hit
TCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTTGCTGCCATTCTCCT19360.136104350418085No Hit
TGCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCAC18820.1323080513878285No Hit
GGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGC18680.13132382571331758No Hit
GGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGGATCTGTG17990.12647299917465646No Hit
AGCTAGAGGATCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTTGCTG17470.12281730381218724No Hit
CTGGTCACCCTGCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGACCC17330.12183307813767631No Hit
CTCCTGGTGGCCCTGCAGGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGA16800.11810708094131343No Hit
GAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGC16500.11599802592450427No Hit
GAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGGATCTG16020.1126235378976096No Hit
GTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATTCTGCAATAGCA15890.11170961405699228No Hit
CCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGT15470.10875693703345946No Hit
GTACGGGGCTCCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGC15380.1081242205284167No Hit
CAGCAGCAGAATGCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCG14830.10425761966426655No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14750.10369520499311745Clontech SMART CDS Primer II A (100% over 21bp)
TATTTGGGATGAGGAAAGGAAATTGAGCAGAAGGTACAGGAGTAATAGCA14580.10250007381692558No Hit
CGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCT14500.10193765914577647No Hit
GCCTGGAGTGGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGC14490.10186735731188283No Hit
GAATACCAGCGTGCATTGCAGGAGAACGTCGCTATGGAACCTGCATCTAC14480.10179705547798919No Hit
AGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAG14360.10095343347126552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)