FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004915658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422438
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT106290.7472381924554884No Hit
CTTCTGGGATGTCCGCTGCAATCTGCTCCGGGGCTGCAGCAACCTCATCA76450.5374575201168698No Hit
TCCCAGAAGTGGTTGTTTCCCTTGCATGGGACGAAAGCTTGGCTCCAAAG62760.4412143095164781No Hit
CTCCTGCAATGCACGCTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTT53690.3774505461749475No Hit
ACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT49830.35031403829200286No Hit
GGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCC36930.2596246725692086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34300.2411352902551816No Hit
GCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTT33840.23790140589607423No Hit
TCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTG33700.23691718022156327No Hit
CTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGCAGATTGCAGC33500.2355111435436905No Hit
TCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTTTCTGCAA32150.22602039596804924No Hit
CTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC32100.22566888679858102No Hit
GGCTGAGCCACTCCAGGCAAGAGCTGATGAGGTTGCTGCAGCCCCGGAGC27790.19536879639042265No Hit
GCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGCGAGGGT26300.18489382314027045No Hit
ATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTG26150.18383929563186585No Hit
AGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAG24050.16907591051420168No Hit
CTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTTGGAGCCAA22460.15789791892511307No Hit
CTGGTATTCTGCAATAGCAGGCCATGTTTTTCCTTGAGCCTGGATGCTTT21920.15410161989485657No Hit
TTGCAGGAGAACGTCGCTATGGAACCTGCATCTACCAGGGAAGACTCTGG21800.1532579978881329No Hit
GAGTACGGGTGCTCCTTGCTATAGAAGACCTGGGACAGAGGACTGCTGTC21670.1523440740475156No Hit
TGCCTGGAGTGGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAG21610.15192226304415377No Hit
TTGCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCA21030.1478447566783227No Hit
GTATCAACGCAGAGTACGGGTGCTCCTTGCTATAGAAGACCTGGGACAGA20940.14721204017327996No Hit
GTATCAACGCAGAGTACGGGGCTCCTTGCTATAGAAGACCTGGGACAGAG20620.1449623814886835No Hit
GCCCAGAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGC20480.1439781558141726No Hit
GAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTT20370.14320483564134254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19870.1396897439466606No Hit
CTTTGGAGCCAAGCTTTCGTCCCATGCAAGGGAAACAACCACTTCTGGGA19840.13947883844497969No Hit
TCTCTGGTCACCCTGCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGA19480.13694797242480866No Hit
GAAGGTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTG19210.1350498229096804No Hit
GCCATGAGGACCCTCGCCATCCTTGCTGCCATTCTCCTGGTGGCCCTGCA18770.1319565422183603No Hit
GCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACC18760.13188624038446667No Hit
CTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGC18730.13167533488278577No Hit
CTCTGGTCACCCTGCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGAC18700.13146442938110484No Hit
GGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATT18420.12949597803208296No Hit
TAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTTTTCTTTT18280.128511752357572No Hit
GGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGG18030.12675420651023103No Hit
GGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCTCATTT17790.12506696249678367No Hit
GGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGCAAGGATGGC17570.12352032215112363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.1216221726359954No Hit
GGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGGATCTGTG16820.11824768460910072No Hit
TCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTTGCTGCCATTCTCCT16720.11754466627016433No Hit
TGCTATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCAC16120.11332655623654599No Hit
CTGGTCACCCTGCCTAGCTAGAGGATCTGTGACCCCAGCCATGAGGACCC15380.1081242205284167No Hit
GTTCCATAGCGACGTTCTCCTGCAATGCACGCTGGTATTCTGCAATAGCA15260.10728059852169304No Hit
TATTTGGGATGAGGAAAGGAAATTGAGCAGAAGGTACAGGAGTAATAGCA15050.10580426000992663No Hit
AGCTAGAGGATCTGTGACCCCAGCCATGAGGACCCTCGCCATCCTTGCTG14810.10411701599647928No Hit
GAGTCTTCCCTGGTAGATGCAGGTTCCATAGCGACGTTCTCCTGCAATGC14770.10383580866090472No Hit
GAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGGATCTG14730.10355460132533018No Hit
CTCCTGGTGGCCCTGCAGGCCCAGGCTGAGCCACTCCAGGCAAGAGCTGA14670.10313279032196834No Hit
GCCTGGAGTGGCTCAGCCTGGGCCTGCAGGGCCACCAGGAGAATGGCAGC14320.10067222613569096No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)