FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004915661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004915661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728728
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT65440.3785442244239695No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT54480.31514500835296244No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC53300.3083191803453175No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG45240.26169530429309873No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT34060.19702347622066627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31840.18418166420628346No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT30980.17920690820071172No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG30530.17660383819779632No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC29970.1733644621941682No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC28380.16416694818386698No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG27160.1571097361759629No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC25530.14768083816540253No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT25120.14530915216274626No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT24080.13929316815600837No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG23920.13836763215497175No Hit
CGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTG22950.13275657014868736No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA21950.12697197014220862No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGAT21930.12685627814207903No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA20050.11598123012989897No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA19610.11343600612704834No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC19120.11060155212387374No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC18420.10655233211933862No Hit
GTATAGACCCGAAACCCGGTGATCTAGCCATGGGCAGGTTGAAGGTTGGG18350.1061474101188851No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG18310.10591602611862594No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG18040.10435418411687669No Hit
TGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGAC17770.10279234211512743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)