FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2356234
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA95060.4034404053247682No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC67010.2843945041112216No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC66730.2832061671294107No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA66390.2817631865086405No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC58780.24946588496728256No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG52200.22153996589472863No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA51130.21699882099995163No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC49440.20982635850259357No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC47030.19959817233772195No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC46440.1970941765546206No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC41940.17799590363266127No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC37200.15787905615486408No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT36770.15605411007565462No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA35790.1518949306393168No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC35560.1509187966899722No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG35310.14985778152764112No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG33860.1437038935861209No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA32250.13687095594070878No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC31760.13479136622253987No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG31730.13466404440306015No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT29640.12579395764597234No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT27690.11751803937978995No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT27580.11705119270836428No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA27420.11637214300447239No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA25850.10970896778503324No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA25530.10835086837724946No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA25130.10665324411751972No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG24490.10393704530195218No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA24360.10338531741754002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57450.061.6975481
GGTATCA33650.056.025521
CAACGCA79250.044.426635
ATCAACG79300.044.3976753
TATCAAC81150.043.8252452
TCAACGC80500.043.7358444
AACGCAG82550.042.938826
TATTCTC26500.040.6212775
TCTCTGC28500.036.9375238
CGCAGAG96000.036.800578
ACGCAGA96200.036.7858857
GCAGAGT100900.035.073869
CTCTGCA31550.034.12229
TTCTCTG33700.031.767427
ATTCTCT36200.030.3937366
AGAGTAC99050.029.09542710-11
GGCGTCG2052.4392648E-929.0125228
GTACATG136600.027.6026151
GTACTGG11000.027.5733281
TACATGG137000.027.1747462
Produced by FastQC (version 0.11.5)