FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409762
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130040.5396383543271078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80430.33376740109604186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61110.2535935084045644No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG34080.1414247548098111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55500.065.906541
GTATCAA118250.054.7738341
CTCAACG5350.053.3622171
CGTATCA4300.047.0280381
ATCAACG150900.044.420263
TCAACGC155450.043.00534
CAACGCA159300.042.0032735
AACGCAG165500.040.4656756
TATCAAC169750.039.697812
CTACATG8150.039.4078331
ACGCAGA185400.036.122277
CGCAGAG188350.035.5565158
GCAGAGT195900.034.2165269
CGACTAT1004.450291E-429.7383161
CCGCAGA7700.029.3521061
GTACTTT92050.027.7843414-15
CGCATGT2006.145092E-826.7644841
AGAGTAC200150.026.13525210-11
CAGAGTA204850.025.81143810-11
GAGTACT102000.025.19036912-13
Produced by FastQC (version 0.11.5)