FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4026046
Sequences flagged as poor quality0
Sequence length125
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251570.6248562485376471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101020.2509161594278853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92140.22885977954548953No Hit
CTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45190.11224412239701186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA190700.071.3259051
GGTATCA65400.066.381871
CTATCAA61250.056.044311
TATCAAC311950.055.4158362
CGTATCA9350.053.9904751
ATCAACG322850.053.387213
TCAACGC326000.053.3703044
CAACGCA326750.053.282135
AACGCAG333600.052.188066
ACGCAGA348150.050.0069967
CGCAGAG357600.048.7011878
GCAGAGT374500.046.3836449
CTACGGG14100.043.758241
AGAGTAC364800.033.59480310-11
AGTACGG252150.031.11961212-13
CAGAGTA374200.030.1952710-11
GAGTACG249200.028.22441512-13
AGTACTT159300.027.81549512-13
GAGTACT156850.027.1235512-13
TACGGGC69000.026.70310414-15
Produced by FastQC (version 0.11.5)