FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816927
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG32470.1787083355577852No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC29670.16329769990759122No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT24410.13434772007901252No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT23720.130550099150929No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG23170.1275230100053552No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG19360.10655353792419837No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA18760.10325125885629967No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC18540.10204042319807015No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT18510.10187530924467521No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.064.125651
GTATCAA54900.055.3499571
ATCAACG69750.043.4829063
CAACGCA70750.043.036425
TCAACGC74400.040.8451584
TATCAAC76850.039.852542
GTACTGG10750.039.8283921
AACGCAG77400.039.799856
CGCAGAG87650.034.9429748
ACGCAGA87700.034.9220927
GCAGAGT89300.034.299229
GCCGTTC1051.4891359E-533.9833768
GGCGTCG2600.032.0227978
AAATACG3450.031.0274475
CATGGGT13700.030.385764
TTCTCTG4950.030.0349857
TAGCGTG800.005374403729.7370939
CAATACG1203.7102393E-529.7346364
ACGGGCC3750.028.547619
ACGGTAC3300.027.0314883
Produced by FastQC (version 0.11.5)