FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590637
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC227460.4954867919201627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189390.4125571244252159No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT133570.2909617989834526No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT100330.2185535471438931No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100260.2184010628590324No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA95950.2090123876054674No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC95190.20735684394126563No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT84590.18426636651950482No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT83770.1824801220397082No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC82560.17984432225854494No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA78740.17152303699900473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77620.16908328844123377No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC74920.1632017517394645No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG62320.1357545804645412No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC61250.1334237492530993No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT59720.13009087845543005No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG57180.12455787726191375No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG55860.12168245931882657No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC54310.11830602158262567No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC53350.1162148085331077No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52120.11353544181341284No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG50150.10924409836804783No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG48850.10641224736349225No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC47010.10240408901858282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA172750.085.603531
GGTATCA104400.058.1979261
TATCAAC258550.057.0410352
ATCAACG274000.053.4835933
ACGCAGA278550.052.769837
CAACGCA278350.052.719245
CGCAGAG277900.052.711948
AACGCAG282800.052.562236
GCAGAGT282200.052.010789
TCAACGC299950.049.07464
ACTGTGC112250.040.785328
CACTGTG103100.040.7045567
CTGTGCA105100.039.929979
CTATTGA48150.035.132159
ATTTAGG52700.034.08861
ACAGCAC125150.033.604853
GCTATTG50800.032.3075688
GGGCTAT54650.031.2170126
ACACAGC133300.030.7338811
GGCTATT55250.030.617437
Produced by FastQC (version 0.11.5)