FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4590637
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC205840.4483909313674769No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT120070.2615541154746063No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT87910.19149847831575445No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA84400.18385248060345438No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC83570.18204445265439195No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT76480.16659997294493117No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC73460.16002136522665592No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA72800.1585836562551123No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT70700.15400912770929176No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC68360.14891179590109171No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG62230.1355585292411489No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT59640.12991661070130353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59150.12884922070727875No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC58220.12682335806555822No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG54990.11978729749270091No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG50790.11063824040105981No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC50170.10928766530657946No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG49240.10726180266485894No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC48630.10593301103964439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150550.085.2501751
GGTATCA92550.058.3360941
TATCAAC226250.056.874452
CGCAGAG232600.055.3884478
ACGCAGA236550.054.9787187
AACGCAG239600.054.6391226
GCAGAGT235700.054.2632069
ATCAACG241250.053.4007533
CAACGCA244450.053.0548175
TCAACGC265850.048.7570274
CACTGTG97050.040.0978247
ACTGTGC106950.039.817528
CTGTGCA100150.038.7861029
ACAGCAC122550.032.9869463
CTATTGA48450.031.47579
TTCTCTG26650.031.309777
ACACAGC128200.030.525531
ATTTAGG53100.030.1616611
GGTCTAT36100.029.5098921
GCTATTG51550.029.1642598
Produced by FastQC (version 0.11.5)