FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004916289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4240337
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA384510.9067911347612231No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC335170.7904324585522329No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA302050.7123254590378076No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC248090.5850714223893054No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG216740.5111386194069009No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC212500.5011394141550541No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC208290.49121095799697057No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC192590.4541855989276324No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA192080.45298286433366025No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT185740.4380312225183989No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA181630.4283385966728588No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC167480.3949686074479457No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC164710.38843610779048926No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAAAAAAAAAAAAAAAAAAAAAAA162410.38301201060198753No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC158690.3742391229753673No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT158650.37414479085034985No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152360.3593110641913603No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC139820.3297379429983985No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA131090.30914995671334616No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC126330.29792443383627293No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA120540.2842698587400011No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC114480.2699785417998617No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA109640.25856435467275357No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC106730.2517016925777361No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT103410.24387212620129015No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT93890.22142108044714373No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA92080.21715255179010537No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG89610.21132754307027954No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA87100.20540820222543632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86200.20328572941254436No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC85700.20210657784982658No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA83850.1977437170677708No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC83710.19741355463020982No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81460.19210737259797983No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG78500.1851267953466906No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT75670.178452797501708No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG73140.17248629059435605No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT71330.16821776193731772No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT69070.16288799687383337No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT68080.1605532767796522No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG67080.15819497365421661No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA66500.156827157841464No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA66190.15609608387257898No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA65250.15387927893466957No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA64610.1523699649343908No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT64050.1510493151841469No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG63030.14864384599620267No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA62730.147936355058572No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG62570.1475590265585023No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC62530.1474646944334849No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC62110.14647420712080195No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT61830.14581388224568No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA59660.14069636446348485No Hit
GTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGAT58410.13774848555669042No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG57480.13555526365003537No Hit
TCATAAAAAGGTCAATCCAACCACCCAAAAAAAAAAAAAAAAAAAAAAAA56890.13416386480602838No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA56460.1331497944620911No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA56030.1321357241181538No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT55940.13192347683686462No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT55910.13185272774310156No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC55430.13072074224289248No Hit
TTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGT53260.12560322446069733No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG52400.12357508377282278No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG51250.12086303517857189No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT49460.11664167258404227No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA49330.11633509317773563No Hit
GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA48850.11520310767752658No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG47120.11112324327052307No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT46480.10961392927024433No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG46400.10942526502020948No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC46340.10928376683268334No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA45870.10817536436372864No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT45730.10784520192616766No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA45570.10746787342609797No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG44780.10560481395700388No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT44640.1052746515194429No Hit
TTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAG44460.10485015695686452No Hit
ATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCA44360.10461432664432095No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA44280.1044256623942861No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA43370.10227960655013976No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG42670.10062879436233488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)