FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004916301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7750192
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGG1104811.425525974066191No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGG489400.6314682268516703No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACCTGTCTCTTATACACATCTC372570.48072357433209395No Hit
TGAAATACAGAGAAAAAGGCCTGTCTCTTATACACATCTCCGAGCCCACG345890.44629862073094445No Hit
TGAAATACAGAGAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGAC343600.4433438552231997No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCACGTACTGCAGTCA238260.30742464186693697No Hit
TGAAATACAGAGAAAAAGGCTGTCTCTTATACACATCTCCGAGCCCACGA237560.30652143843662194No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCCGTACTCTGCGTT206580.2665482351921088No Hit
TGAAATACAGAGAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACC170320.2197622975017909No Hit
TCATAAAAAGGTCAATCCAACCACCCAAATCAAGCGTTACGTACTGCAGT139730.1802923075970247No Hit
TGAAATACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAGAGA125790.1623056564276085No Hit
TCATAAAAAGGTCAATCCCTGTCTCTTATACACATCTCCGAGCCCACGAG118410.15278331169085876No Hit
TGAAATACAGAGAAAAAGGCATCCCTGTCTCTTATACACATCTCCGAGCC112760.14549316971760184No Hit
TCATAAAAAGGTCAATCCAACCACTGTCTCTTATACACATCTCCGAGCCC107430.13861592074106036No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCCGTACCTGTCTCT98570.12718394589450172No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCTGTCTCTTATACAC91080.11751966919013102No Hit
TCATAAAAAGGTCAATCCAACCACCCAAATCAAGCGTTACGTACTAGCAT85000.10967470225253775No Hit
TGAAATACAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCAG79280.10229423993624931No Hit
TGAAATACAGAGAAAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAG78110.1007845999170085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)