FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349317
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC23680.10079525240740181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61050.055.043071
GGTATCA31850.054.062691
ATACGGG2300.044.0382771
ATCAACG83550.042.6231773
TATCAAC84700.042.0444682
TCAACGC86150.041.4058234
AACGCAG89800.039.789056
CAACGCA89750.039.7449765
CGCAGAG95250.037.574828
ACGCAGA95100.037.571577
GCAGAGT106600.033.5183569
AAGTACG2201.5643309E-1029.7906021
TACGATC1602.6234557E-729.725937
AGAGTAC101050.029.15229610-11
CCGTGCG1358.295334E-526.4230489
AGTACGG91900.026.29725612-13
CGTACCG1150.001009884625.8486335
ACTGTGC24250.025.0065548
CAGAGTA109250.024.70585310-11
GCGTTAC2550.024.52715934-35
Produced by FastQC (version 0.11.5)