FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298902
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC38850.2990987772749599No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA38070.29309370529878315No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA37410.28801249054971045No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC29230.22503622290211273No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG25610.19716652988447164No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC24970.19223929133991632No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC23400.18015215928530404No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA21660.16675622949229427No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC21320.16413863401549925No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC20550.1582105501415811No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT20360.15674777619866626No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC18500.14242798917855234No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC18370.14142714384918956No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA18230.14034931041756807No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC17060.13134170245330287No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA17010.13095676194200948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16330.12572157098841946No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC16100.12395084463646987No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA14870.11448130805865261No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT14570.11217166499089232No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC14030.10801430746892374No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA13410.10324104512888577No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA13350.10277911651533372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27350.085.212761
GTATCAA59100.080.470681
TATCAAC83100.057.353232
AACGCAG90650.053.217946
ATCAACG90450.053.011073
ACGCAGA92450.052.1019027
CGCAGAG92850.051.8774458
GCAGAGT92650.051.523169
TCAACGC93600.051.2270434
CAACGCA94500.050.973615
TTTAGGG16100.036.2209662
ACTGTGC11700.035.6924638
ATTTAGG16200.035.5160031
TATGGTA4150.032.961462
CACTGTG12300.032.780637
CTGTGCA13250.032.6039169
TTAGGGC19600.032.324183
CTATTGA18250.031.95639
TCTATAC7050.031.6572723
GCTATTG18850.030.9391258
Produced by FastQC (version 0.11.5)