FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5392523
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91890.17040261117106037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84260.1562533901107144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA158500.048.192021
GGTATCA85600.042.7406121
ATATCAA53850.041.7585531
TATCAAC260500.037.452042
ATCAACG294600.036.832522
CAACGCA296600.036.624614
TCAACGC296400.036.589113
AACGCAG309500.035.1365365
ATACATG68700.033.1651081
AGTATCA44450.031.0495681
AAGTACA46050.029.5832041
ACGCAGA386300.028.212676
CGCAGAG388500.028.022547
GCAGAGT413950.026.4721458
CAGAGTA421750.026.0671949
AGAGTAC411950.025.53178210-11
TAGACCG3051.0913936E-1125.3563585
AGTACTT176200.023.98841712-13
ACCGTTA5000.022.6063798
GAGTACA319300.022.3389976
Produced by FastQC (version 0.11.5)