FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5392523
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA148600.052.8862461
GGTATCA83950.044.3621251
TATCAAC194100.039.9364622
CAACGCA197700.038.818415
ATCAACG200300.038.5517773
TCAACGC201100.038.280084
AACGCAG205000.037.4036066
ACGCAGA240500.031.9555057
CGCAGAG240800.031.8389288
GTACATG383850.030.951041
TACATGG377450.030.7817612
ACATGGG367850.030.3559763
CATGGGG138700.029.7669054
GCAGAGT263150.029.1799729
AGAGTAC254050.025.98685310-11
CATGGGA138650.025.9588934
CATGGGT53100.024.6478374
CATGGGC95700.023.4358164
AGTACAT290700.022.4229812-13
CAGAGTA264000.021.56045510-11
Produced by FastQC (version 0.11.5)