FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4174700
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT76630.18355809998323233No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG64350.15414281265719693No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG53270.12760198337605097No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA46050.11030732747263276No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT45330.1085826526456991No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT43850.10503748772366878No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT43760.10482190337030206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA264000.044.8796231
GTACAAA6800.041.11051
ATCAACG340900.034.406343
TCAACGC343050.034.1713184
CAACGCA344250.034.0328835
GGTATCA167700.033.3039931
TATCAAC357800.033.013952
AACGCAG355350.032.915256
TATACGA13450.031.8396872
TACACAG10700.031.1250975
ACGCAGA405700.028.7511067
CGCAGAG406900.028.5925778
ATACGAC15700.027.6555183
ATGGGAG53800.027.6353025
GATCTAC22500.027.4925231
ATATACG15500.027.245231
GCAGAGT442650.026.417969
GACACTC17700.025.5270677
CATGGGT89500.025.3182954
CATGGGG139550.024.5911314
Produced by FastQC (version 0.11.5)