FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004916331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61830
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG11671.8874332848131976No Hit
NTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT9611.554261685266052No Hit
NAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT7981.2906356137797186No Hit
NACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC7891.2760795730228045No Hit
NACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG7211.1661005984150088No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG6471.0464175966359373No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT6180.9995147986414363No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT4700.7601487950832929No Hit
AACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCG4170.6744298884036876No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC3930.6356137797185832No Hit
NCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1630.2636260714863335No Hit
NTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC1450.23451398997250528No Hit
NGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1440.23289665211062593No Hit
NAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA1190.19246320556364224No Hit
CTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTC1110.17952450266860748No Hit
NTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1100.1779071648067281No Hit
NTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC1060.17143781335921074No Hit
NGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT1000.16173378618793466No Hit
NACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC970.15688177260229663No Hit
NTGAAAACTTCCGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCAT940.15202975901665858No Hit
NACCGCCTGCGAGCCCTTTACGCCCAATAATTCCGGACAACGCTTGCCAC930.15041242115477924No Hit
NGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA930.15041242115477924No Hit
NCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC890.14394306970726184No Hit
NGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC890.14394306970726184No Hit
NGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC860.1390910561216238No Hit
NATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA780.12615235322658902No Hit
AAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGA770.12453501536470969No Hit
NGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA760.12291767750283035No Hit
NGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG760.12291767750283035No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG740.11968300177907165No Hit
CTACGCATTTCACCGCTACACCTGGAATTCTACCCCCCTCTACGAGACTC710.1148309881934336No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC710.1148309881934336No Hit
NGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCCCCAGT710.1148309881934336No Hit
NGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCAT700.11321365033155426No Hit
NACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCC690.11159631246967493No Hit
NCACCAGTCCTTCCGGTCTGGCTTCACAGCCCTTAGAACGCTCTCCTACC680.10997897460779556No Hit
NATCACTCGCAGTCTGACTCCCAAGGATAAGTCATTGGCATTCGGAGTTT670.10836163674591621No Hit
NAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT670.10836163674591621No Hit
NGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC660.10674429888403687No Hit
NTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT640.10350962316027817No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC640.10350962316027817No Hit
NCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTGCGTCCC640.10350962316027817No Hit
TACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTC630.10189228529839883No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)