FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004916332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61830
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2320.37522238395600843No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1210.19569788128740093No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1090.17628982694484877No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA1090.17628982694484877No Hit
TGGTGGTAGTAGCAAATATTCCAACGAGAACTTTGAAGGCCGAAGTGGAG820.13262170467410642No Hit
TGGTGGNAGTAGCANATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG810.13100436681222707No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG740.11968300177907165No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG720.11644832605531297No Hit
GGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGG710.1148309881934336No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA680.10997897460779556No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA670.10836163674591621No Hit
GGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCCCG640.10350962316027817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)