FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065384
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC43990.4129027655756047No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT24170.2268665570348344No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT20480.19223115796745588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19760.1854730313201625No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG17390.16322753110615515No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT16830.15797121038048254No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT14410.1352563958159687No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT13840.1299062122201948No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC13140.123335811313104No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG13080.12277263409249622No Hit
GTCTCGGGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGG12700.11920584502864695No Hit
GTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGCCCGGTGCCGG11590.10878706644740299No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG11580.1086932035773017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.10737912339588356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.100245545268185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89650.070.389921
GGTATCA51750.065.240641
TATCAAC124600.050.5992852
ATCAACG133500.047.279913
TCAACGC138000.045.8946344
CAACGCA149500.042.7494055
AACGCAG150600.042.5805326
CGCAGAG152150.042.4305658
ACGCAGA152650.042.2444347
GCAGAGT153100.041.7912039
ACTGTGC23050.039.9666148
CTGTGCA22450.038.4700939
CACTGTG23750.036.9704327
TATAGCG1051.9352867E-434.271977
AGCACTG26700.032.3465735
ACACAGC27050.031.3372761
TCTATAC9200.031.2917943
GTCTATA10250.030.1927662
ATCACTC4800.029.9879688
TAGTATA4800.029.9879684
Produced by FastQC (version 0.11.5)