FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4142489
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG426171.0287776262049217No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT253090.6109611878269321No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC183490.44294625767262147No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC159760.38566185691742333No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA140430.33899908967772757No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA138070.33330203170123085No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT109220.2636579119461753No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG84550.20410434403084718No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT78080.1884857147478243No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT77280.18655450865409662No Hit
GGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTT71670.17301192592183104No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC63190.15254114132831734No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA61470.14838904822680277No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA60670.14645784213307506No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG58930.1422574688792173No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG56790.1370914925784957No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC55620.13426710366641892No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG53460.1290528472133541No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG51270.1237661705317745No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT48610.11734491027012986No Hit
CGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATT42260.10201596190116619No Hit
GGATTAGCCAACCTTCGTGCTCCTCCGTTACTCTTTAGGAGGAGACCGCC41450.10006061573126687No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCCG61800.053.0986061
CGACTCC62950.052.1979836
ACTCCCT63550.051.5563438
GCGACAT4850.050.9102135
CCGACTC65750.050.1195375
TGCGACA5200.046.8941844
GACTCCC71000.046.3466387
CTGCGAC5550.045.6599123
GTAGCAA35950.045.040599
CTCCCTT73300.044.8281369
AGTAGCA36650.044.4394538
TAGAGCC38050.044.426157
TTAGAGC37950.044.418096
TAGTAGC38500.041.9335377
ACCCGAC78550.041.878823
AACCCGA79450.041.769562
CCCGACT79850.041.5562824
CGGCCTA20350.037.333979
GTAGTAG35000.035.4091726
AGAGCCA49400.034.603838
Produced by FastQC (version 0.11.5)