FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004916358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004916358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3395715
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155200.45704660137850206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77210.2273747944100138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72930.2147706742173592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40940.12056371044095279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA173150.073.7713551
GGTATCA94300.067.0330051
CAACGCA250200.051.9077535
TATCAAC252200.051.324842
ATCAACG261250.049.547623
AACGCAG262700.049.327496
TCAACGC267350.048.6318244
ACGCAGA267300.048.103687
CGCAGAG265850.048.0952458
GCAGAGT275600.045.8713079
CAGAGTA275050.026.8030439
TAGTACT21900.025.9696454
TACTGTG24200.025.2871197
ACTGTGC31450.024.9517888
TAGGCAT26200.023.6308965
CTGTGCG22950.023.5274269
TTTAGGG25100.022.9455932
CTATTGA23350.022.5077349
ATTTAGG24100.022.0590321
CTAGGCA32100.021.9788044
Produced by FastQC (version 0.11.5)